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Extraction of DNA from E coli
14:43
YouTubeProfessor Drew Collop
Extraction of DNA from E coli
Demonstration of the extraction of DNA from E.coli. Cells were harvested, pelleted and diluted in TE Buffer pH 8.0 [0.15 M NaCl; 1 mM EDTA; 10 mM Tris]. 1 mL of 10 mg/mL Lysozyme was added to break down the cell wall. 2 mL of 20 % (w/v) SDS was added to dissolve the exposed plasma membrane and lyse the cell. NaCl was added to a final ...
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TE Buffer Recipe
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